rabbit anti tubb3  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

doi: 10.64898/2026.03.30.715210

Figure Lengend Snippet: a , Schematic illustration for identification of Creld1-interacting protein in DRGs by immunoprecipitation and mass spectrometry. b , Representative Na v currents in DRG neurons from control and sgCreld1 mice. c , Representative Na v currents recorded in DRG neurons derived from WT and cKO mice. d , Scatterplot of normalized mRNA levels of the indicated Na v isoforms expressed in WT and cKO DRGs. e , Immunofluorescent staining of either Na v 1.7 and TUBB3 from the indicated DRG sections. Scale bar, 20 μm. f , Total density of Na v 1.7 expression of DRG neurons from WT (389 cells) and cKO (252 cells). g , Immunofluorescent staining of endogenous Creld1 and Na v 1.7 in DRG neurons after 36 h in culture using the anti-Creld1, anti-Na v 1.7 and anti-TUBB3 antibody. Scale bars, 100 μm (top); 5 μm (bottom). h , Pearson’s co-localization efficiency analysis for Na v 1.7 and Creld1 located in DRG neurons after 36 h in culture. The white circle illustrates the region of interest (ROI) used for analyzing the immunofluorescence staining of Na v 1.7 and Creld1. Each dot represents an individual cell (17 cells in total).

Article Snippet: Primary antibodies: rabbit anti-GFP (ThermoFisher Scientific, A11122, 1:500 dilution), chicken anti-GFP (Aves Labs, GFP-1020, 1:500 dilution), chicken anti-NFH (Aves Labs, NFH, 1:500 dilution), biotin IB4 (ThermoFisher Scientific, I21414, 1:100 dilution), rabbit anti-CGRP (immunostar, 24112, 1:500 dilution), mouse anti-Piezo2 (generated in our lab, 1:500 dilution), mouse anti-Trpv1 (Abcam, ab203103,1:500 dilution), human anti-Na v 1.7 (US11643458B2, a gift from Dr. Juanjuan Du at Tsinghua university, 1:500 dilution), goat anti-Creld1 (R&D systems, AF4116, 1:500 dilution), rabbit anti-TUBB3 (CST, 5666S, 1:500 dilution), rabbit anti-PGP9.5 (Cell signaling technology, 13179).

Techniques: Immunoprecipitation, Mass Spectrometry, Control, Derivative Assay, Staining, Expressing, Immunofluorescence

Journal: bioRxiv

Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1

doi: 10.64898/2026.03.28.714577

Figure Lengend Snippet: ( A ) Western blot analysis of subcellular fractions from HDF treated for 6 days with doxycycline (Dox) to induce progerin expression, for 24 h with hydroxyurea (HU), or transfected with single-stranded DNA (ssDNA). Fraction markers include β-tubulin (cytoplasm), SEC61 (membrane), Lamin A (nucleus), and Histone H3 (chromatin). Note the increased presence of STING at nucleus and chromatin upon replication stress. Membranes imaged with prolonged exposure (high exposure) show a marked increase in signal intensity. ( B ) Immunofluorescence (IF) with STING antibody in HDF treated with vehicle, ssDNA, HU, Doxy (progerin), or dsDNA. ( C ) Quantification of cGAMP levels measured by ELISA in HDF treated as indicated. ( D ) IF with STING antibody and quantification of percentage of cells showing STING localization to the perinuclear compartment (PNC) upon different treatments. ( E ) IF with antibody recognizing phosphorylated STING on Ser366 and quantification of percentage of cells positive for S366 p-STING. ( F ) Immunoblot analysis of STING, GFP-progerin, and markers of activation of the canonical cGAS-STING pathway ( S366 p-STING, S386 p-IRF3, and S172 p-TBK1) following treatments. ( G ) Immunoblot analysis of STING pathway components and ISG proteins (STAT1, S727 p-STAT1, RIG-I, and ISG15) after indicated treatments.

Article Snippet: Primary antibodies used were β-tubulin (1:5000- Origene-AP31823PU-N), GAPDH (1:1000- Cell Signaling-2118), Lamina A (1:3000- Abcam-1791), Progerin (1:1000 Santa Cruz-81511), ISG15 (1:1000 Santa Cruz-166755), STING (1:1000-Cell Signaling-13647), S366 p-STING (1:1000- Cell Signaling- 50907), RIG-I (1:1000-Cell Signaling-3743S), S33-p-RPA (1:1000 Bethyl- PLA0070), SAMHD1 (1:1000- Cell Singaling-49158), λH2AX (1:1000- Cell Signaling- 2577).

Techniques: Western Blot, Expressing, Transfection, Membrane, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: bioRxiv

Article Title: The RNA and protein landscapes of mouse brain organoids

doi: 10.64898/2026.03.13.711293

Figure Lengend Snippet: (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

Article Snippet: The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).

Techniques: Transcriptomics, Immunostaining, Derivative Assay, Staining